What is the correct order of components to be added when labeling red blood cells with radiochromium?

When labeling red blood cells with radiochromium, the correct order of components ensures optimal labeling efficiency and integrity of the blood sample. The process typically begins with patient blood, which is collected into a suitable container. The next step is to add radiochromium to the patient blood. This sequence allows the radiochromium to effectively label the red blood cells as they are in the medium where the labeling reaction takes place.

Following the addition of radiochromium, ascorbic acid is introduced. Ascorbic acid serves to enhance the labeling process by reducing oxidation, which can affect the efficiency of labeling and the viability of the red blood cells. This is critical as preserving the integrity of the red blood cells is necessary for the accuracy of subsequent imaging or diagnostic procedures.

By understanding this sequence—starting with patient blood, then adding radiochromium, and finally incorporating ascorbic acid—professionals can ensure that the radiolabeling procedure is set up correctly, facilitating high-quality diagnostic imaging outputs.